Giuseppina Andreotti

I obtained a degree in Chemistry (with a focus on organic-biological chemistry) in 1990 from the University of Naples Federico II. In 1996, at the same university, I earned a PhD in Biochemical Sciences.

In 1996, I began working at the Institute for the Chemistry of Molecules of Biological Interest of the CNR in Arco Felice, Naples (CNR-ICMIB), which was renamed the Institute of Biomolecular Chemistry (CNR-ICB) in 2001. Since December 2001, I have held a permanent research position at CNR-ICB, and since January 2023, I have been a Senior Researcher.

My research activity began with the study of enzymes from extremophilic organisms (Sulfolobus solfataricus, Pyrococcus furiosus, Thermococcus litoralis). I then expanded my interests to the application of Nuclear Magnetic Resonance (NMR) spectroscopy—initially for food characterization and subsequently for structural studies of proteins and peptides. Later, I focused on glycosyl hydrolases from marine organisms.

Since 2010, I have concentrated on certain rare genetic diseases and therapeutic approaches using pharmacological chaperones (PCT, pharmacological chaperone therapy) or, more generally, small molecule therapy (SMT, small molecule therapy). My main focus is on:

· Fabry disease, associated with mutations in the GLA gene (lysosomal alpha-galactosidase), and

· Congenital disorders of glycosylation related to phosphomannomutase 2 deficiency, due to mutations in the PMM2 gene.

My main objectives are to elucidate the molecular mechanisms underlying these diseases and to develop personalized therapeutic approaches.

1. M. Monticelli, D. Paris, M.C. Monti; E. Morretta, Z. Pakanova, M. Nemcovic, R. Kodrikova, M.V. Cubellis; G. Andreotti* Beneficial effects of Glc-1,6-P2 modulation on mutant phosphomannomutase-2, BBA – Molecular Cell Research, 2025, https://doi.org/10.1016/j.bbamcr.2025.119948
2. Monticelli, M, Hay Mele, B.; Wright, D.M., Guerriero, S., Andreotti, G.*, Cubellis, M.V. Exploring Ligand Interactions with Human Phosphomannomutases Using Recombinant Bacterial Thermal Shift Assay and Biochemical Validation. Biochimie 2024, doi: 10.1016/j.biochi.2024.02.011
3. M Monticelli, DM Wright, MV Cubellis, G Andreotti*. ReBaTSA: A simplified CeTSA protocol for studying recombinant mutant proteins in bacterial extracts. BBA-GeneralSubjects1868(2024)130526
https://doi.org/10.1016/j.bbagen.2023.130526
4. Monticelli M, Hay Mele B, Allocca M, Liguori L, Lukas J, Monti MC, Morretta E, Cubellis MV, Andreotti G. Curcumin Has Beneficial Effects on Lysosomal Alpha-Galactosidase: Potential Implications for the Cure of Fabry Disease. IJMS 2023; 24(2):1095. https://doi.org/10.3390/ijms24021095
5. Vignogna RC, Allocca M, Monticelli M, Norris JW, Steet R, Perlstein EO, Andreotti G*, Lang GI*. Evolutionary rescue of phosphomannomutase deficiency in yeast models of human disease. eLife 2022;11:e79346. DOI: https://doi.org/10.7554/eLife.79346

   MAIN RESEARCH SITE, POZZUOLI (NA)

   giuseppina.andreotti@cnr.it

   PEC: gandreotti@pec.it

   (+39) 081 8675241

ORCID: https://orcid.org/0000-0002-1594-0156

Scopus Author ID:
7004848704

Web of Science ResearcherID: AAX-8952-2020