Marco Allarà

Marco Allarà was born in Naples on 10-11-1986, he began working at ICB-CNR in Pozzuoli in 2006. At the end of 2009 he was hired by the Epitech Group company and continued his activity in the field of cellular biology. In 2012 he obtained a degree in Industrial Biotechnology, discussing a thesis on the metabolism of PEA. Over the years he participated in the management and organizational activities of the ICB and acquired skills in various fields such as: ANALYTICAL CHEMISTRY, LC-MS and LC-MS/MS analysis of small molecules. Management and maintenance of instruments, Extraction of biomolecules from human and animal fluids and tissue, Purification of bioactive lipids on silica gel, Enzyme activity assays using radiolabeled precursors, Binding assays
ORGANIC CHEMISTRY: Phospholipid synthesis of N-acylphosphatidylethanolamines Synthesis of radiolabeled and non-radiolabeled diacylglycerols (DAG) Medium and long chain N-acylethanolamines Synthesis of N-acyl amino acids
MOLECULAR BIOLOGY Extraction of proteins from different types of cells and tissues Purification of recombinant proteins.
In 2020 he obtained a Master’s Degree in Drug Biotechnology, discussing a thesis on the role of PEA in neuroinflammation. In March 2025 he was hired by the ICB in the role of CTER VI level.

Gestione e manutenzione delle strumentazioni

Estrazione di biomolecole da fluidi e tessuto umano e animale

Protocollo

Purificazione su gel di silice di lipidi bioattivi

Saggi di attività enzimatica con utilizzo di precursori radiomercati

Inventario

Saggi di binding

Trasfezione di DNA plasmidico codificante per i recettori CB1 e CB2 e per il recettore vanilloide TRPV1

Estrazione del DNA/RNA e di proteine da differenti tipi di cellule e tessuti

Purificazione di proteine ricombinanti

Allestimento e mantenimento di colture cellulari sia primarie che immortalizzate

Vitalità, antiproliferazione e citotossicitàapoptosi

Proliferazione e differenziamento cellulare

– De Petrocellis L, Ligresti A, Moriello AS, Allarà M, Bisogno T, Petrosino S, Stott CG, Di Marzo V. “Effects of cannabinoids and cannabinoid-enriched Cannabis extracts on TRP channels and endo-cannabinoid metabolic enzymes.” “Br J Pharmacol. 2011 Aug SCOPUS

– Baggelaar MP, Janssen FJ, van Esbroeck AC, den Dulk H, Allarà M, Hoogendoorn S, McGuire R, Florea BI, Meeuwenoord N, van den Elst H, van der Marel GA, Brouwer J, Di Marzo V, Overkleeft HS, van der Stelt M. “Development of an Activity-Based Probe and In Silico Design Reveal Highly Selec-tive Inhibitors for Diacylglycerol Lipase-α in Brain” Angew Chem Int Ed Engl SCOPUS

– Baggelaar MP, Chameau PJ, Kantae V, Hummel J, Hsu KL, Janssen F, van der Wel T, Soethoudt M, Deng H, den Dulk H, Allarà M, Florea BI, Di Marzo V, Wadman WJ, Kruse CG, Overkleeft HS, Hankemeier T, Werkman TR, Cravatt BF, van der Stelt M. Highly Selective, Reversible Inhibitor Iden-tified by Comparative Chemoproteomics Modulates Diacylglycerol Lipase Activity in Neuronens. J Am Chem Soc. SCOPUS

– 12. Annelot C. M van Esbroeck, Antonius P. A. Janssen, Armand B. Cognetta, Daisuke Ogasawara, Guy Shpak, Mark van der Kroeg, Vasudev Kantae, Marc P. Baggelaar, Femke M. S. de Vrij, Hui Deng, Marco Allarà, Filomena Fezza, Zhanmin Lin, Tom van der Wel, Marjolein Soethoudt, Elliot D. Mock, Hans den Dulk, Ilse L. Baak, Bogdan I. Florea, Giel Hendriks, Luciano De Petrocellis, Herman S. Overkleeft, Thomas Hankemeier, Chris I. De Zeeuw, Vincenzo Di Marzo, Mauro Maccarrone, Benja-min F. Cravatt, Steven A. Kushner, Mario van der Stelt “Activity-based protein profiling reveals off-target proteins of the FAAH inhibitor BIA 10-2474” SCOPUS